* Sample Name
For multiple samples, use "," to separate. e.g. "Sample01,Sample02,Sample03,...Sample10" or "Sample 1-10". You can also attach the sample list in the end.
Cap Name
If you want the name on the cap to be different from the sample name, or the sample name is longer than 20 characters, please enter the cap name here.
* Sample Quantity
-- Please Select -- 1 sample > 1 sample (enter Qty. at the end of this page)
e.g. If you have five samples, enter number 5 in the Qty box at the end of this page.
* Endotoxin Free
-- Please Select -- Yes (< 0.1 EU/µg DNA) +$20.00 No (~9.3 EU/µg DNA)
Endotoxins are cell membrane components of Gram-negative bacteria (e.g., E. coli). Endotoxins are often co-purified with plasmid DNA and can significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. Typical applications such as sequencing and cloning are not affected by endotoxins.
* Starting Material
-- Please Select -- DNA in water or buffer +$20.00 DNA on filter paper +$30.00 Bacterial Colony Glycerol Stock Bacterial Stab
* Host Strain
-- Please Select -- DH5-Alpha Top10 DH10B Stbl2* Stbl3* NEB Stable* EC100D pir+* Other
Please select the host strain of your bacterial stock or the strain that you'd like to perform the transformation with. If you select "other", please provide the bacterial stock or competent cells when sending your samples.
* Antibiotic
-- Please Select -- Amp/Carb Kan Chlor Other
Other Antibiotic
Please supply sufficient antibiotic for at least 1.0 L of bacterial culture.
* Copy Number
-- Please Select -- High Copy (>300 plasmids / cell) Medium Copy (100-300 plasmids / cell) Low Copy (20-100 plasmids / cell) Very low copy (<20 plasmids / cell) Unknown
Plasmid Backbone
We highly recommend you provide the backbone information. It may help us improve your production.
* Terminal Repeat
-- Please Select -- No Contains ITRs Contains LTRs Contains other TRs Unknown
ITR: Inverted Terminal Repeat from adeno-associated viruses (AAV)
* Size (bp)
Estimated plasmid size in base-pair
Final Buffer
-- Please Select -- TE buffer Water 1xPBS
PLASMID QUALITY CONTROL OPTIONS (Scroll down to the end if no additional QC needed)
* Sequencing
-- Please Select -- No Sequencing Whole Plasmid Sequencing (no primer needed) +$17.00 1X Sanger Sequencing +$10.00 2X Sanger Sequencing +$20.00 3X Sanger Sequencing +$30.00 4X Sanger Sequencing +$40.00 5X Sanger Sequencing +$50.00
Primer
-- 3GEX 5GEX BGHR CMV-F pBAD-F EGFP-C EGFP-N M13F(-21) M13F(-41) M13F(-47) M13R Myc Neo-F Neo-R pBAD-R pFastBacF pFastBacR SP6 SV40-promoter SV40pA-R T3 T7 T7-EEV T7-Term V5
Hold down the Ctrl key (Command key on OS X) while selecting multiple primers.
Your Primer(s)
Please supply your primer(s) with your sample
* Restriction Analysis
-- Please Select -- No digestion needed 1X Digestion +$20.00 2X Digestions +$40.00 3X Digestions +$60.00
Restriction Enzyme(s)
-- AgeI AhdI ApaI ApaLI AsiSI AvrII BamHI BglI BglII BspQI BstBI ClaI EagI EcoRI EcoRV HindIII HpaI KpnI MluI NcoI NdeI NheI NotI PstI PvuI PvuII SacI SalI ScaI SgfI SmaI SpeI SphI XbaI XcmI XhoI XmaI
Hold down the Ctrl key (Command key on OS X) while selecting multiple enzymes .
Digestion Plan
Please describe your digestion plan. e.g. Sample01/BamHI + XhoI: 3642bp + 1527bp
Sterility Test
-- Please Select -- Sterility Test +$20.00 0.22um Filtration +$20.00 0.22um Filtration + Sterility Test +$40.00
Supercoiled pDNA Analysis
-- Please Select -- Supercoiled pDNA analysis +$20.00 Extra supercoiled treatment +$30.00 Extra supercoiled treatment + supercoiled pDNA analysis +$50.00
The percentage of supercoiled pDNA will be analyzed from an agarose gel image by electrophoresis evaluation software.
Endotoxin Test
-- Please Select -- Endotoxin test +$150.00 Extra endotoxin removal +$150.00 Extra endotoxin removal + LAL endotoxin test +$300.00
Extra endotoxin removal with Triton-X114 method will further reduce the endotoxin level.
Concentration Normalization
+$10.00
Plasmid DNA concentration of the final product will typically range between 1-4 mg/mL. To request a specific concentration (between 0.1 to 5 mg/ml) please enter the concentration in the field above. Leave blank if no concentration normalization is required. SAMPLE STORAGE
* Glycerol Stock
-- Please Select -- No Save new glycerol stock at Alta Biotech +$10.00 Ship new glycerol stock back +$10.00
**If you are choosing “Ship glycerol stock back”, please select the “Fedex Dry Ice Overnight” shipping option when checking out.
* Original & QC-testing Sample Storage
-- Please Select -- Destroy original & QC-testing sample(s) upon completion Save original & QC-testing sample(s) at Alta Biotech for 12 months
Comments
Additional File
