DNA Prep
Alta Biotech's experienced DNA Prep scientists provide fast and efficient plasmid DNA purification. We select commercial anion-exchange kits from premium brands to prepare pure plasmid DNA. Our in-house developed semi-automated process and enhanced quality control procedures reduce human errors and cost. Alta Biotech is committed to delivering high-quality plasmid DNA for cell transfection, transgenic injection, DNA vaccine development, and other applications.
We provide flexible services options for the DNA prep: DNA Prep scale from Mini to Giga, or targeted amount
Specialized DNA prep protocols applied to both high and low-copy plasmids to optimize yield and quality. DNA prep applied for high-copy plasmids (e.g. pUC, pGEM, pTZ, pBluescript derived vector with pUC, F1 origin or derivative pMB1 or ColE1 origin) or low-copy plasmids (e.g. plasmid with wild type pMB1 or ColE1 origin, pBR322, R6k, or p15A origin)
Endotoxin-free prep (< 0.1 EU/µg DNA)*, or Non-endotoxin-free prep (~9.3 EU/µg DNA) services are available
We accept starting materials from plasmid DNA*, bacterial colony, bacterial stab, bacterial pellet, and glycerol stock
Standard quality analysis for plasmids using OD260/280 and OD260/230. Optional extra QC of sanger sequencing, restriction enzyme digestion, and sterile test are also available*
Final product concentration normalization and custom aliquots*
Quick turn-around times in 2-4 business days (bulk orders may affect turn-around times)
* Additional charges apply
For bulk or custom orders, download and complete the Plasmid DNA Prep Request Form and email to [email protected] or fax to +1 800-878-3281. We will contact you within 1 business day after we receive your request.
Form
We provide flexible services options for the DNA prep:
* Additional charges apply
For bulk or custom orders, download and complete the Plasmid DNA Prep Request Form and email to [email protected] or fax to +1 800-878-3281. We will contact you within 1 business day after we receive your request.
Form
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eXPRESS Giga Prep
SKU:A101
$370.00eXPRESS Giga Prep service isolates up to 15mg of ultrapure plasmid DNA. We guarantee 10mg for high-copy plasmids. Turnaround time is 3-5 business days (bulk orders may affect turn-around times).
Expected Yields:
Learn MoreCopy Number (Copies per cell) Typical Yield (mg) High-copy (> 300) 10mg - 13mg Medium-copy (100 - 300) 5mg - 8mg Low-copy (20 - 100) 2mg - 5mg Very low-copy (< 20) 1mg - 2mg -
eXPRESS Mega Prep
SKU:A102
$210.00eXPRESS Mega Prep service isolates up to 5mg of ultrapure plasmid DNA. We guarantee 3mg for high-copy plasmids. Turnaround time is 3-4 business days (bulk orders may affect turn-around times).
Expected Yields:
Learn MoreCopy Number (Copies per cell) Typical Yield (mg) High-copy (> 300) 3mg - 4mg Medium-copy (100 - 300) 1.5mg - 2.5mg Low-copy (20 - 100) 0.5mg - 1.0mg Very low-copy (< 20) 0.2mg - 0.5mg -
eXPRESS Maxi Prep
SKU:A103
$100.00eXPRESS Maxi Prep service isolates up to 1.5mg of ultrapure plasmid DNA. We guarantee 1mg for high-copy plasmids. Turnaround time is 3-4 business days (bulk orders may affect turn-around times).
Expected Yields:
Learn MoreCopy Number (Copies per cell) Typical Yield (ug) High-copy (> 300) 1000ug - 1300ug Medium-copy (100 - 300) 500ug - 800ug Low-copy (20 - 100) 200ug - 500ug Very low-copy (< 20) 100ug - 200ug -
eXPRESS Midi Prep
SKU:A104
$75.00eXPRESS Midi Prep service isolates up to 500ug of ultrapure plasmid DNA. We guarantee 300ug for high-copy plasmids. Turnaround time is 3-4 business days (bulk orders may affect turn-around times).
Expected Yields:
Learn MoreCopy Number (Copies per cell) Typical Yield (ug) High-copy (> 300) 300ug - 500ug Medium-copy (100 - 300) 150ug - 250ug Low-copy (20 - 100) 50ug - 150ug Very low-copy (< 20) 20ug - 50ug -
eXPRESS Mini Prep
SKU:A105
$15.00eXPRESS Mini Prep service isolates up to 30ug of ultrapure plasmid DNA. We guarantee 15ug for high-copy plasmids. Turnaround time is 3-4 business days (bulk orders may affect turn-around times).
Expected Yields:
Learn MoreCopy Number (Copies per cell) Typical Yield (ug) High-copy (> 300) 15ug - 30ug Medium-copy (100 - 300) 5ug - 15ug Low-copy (20 - 100) 1ug - 5ug Very low-copy (< 20) Not recommended
