Sub-cloning

Project Name

Maximum number of characters: 50

*Insert Name

Maximum number of characters: 30

*Insert Donor Vector

Maximum number of characters: 30

*Destination Vector

Maximum number of characters: 30

*REs on Destination Vector

-- AgeI ApaI BamHI BglII BstBI ClaI EcoRI EcoRV HindIII Klenow KpnI MluI NcoI NdeI NheI NotI PstI PvuII SacI SalI SmaI SpeI XbaI XhoI

RE: Restriction Enzyme

*REs on Insert

-- ApaI BamHI BglII BstBI ClaI EcoRI EcoRV HindIII Klenow KpnI MluI NcoI NdeI NheI NotI PstI PvuII SacI SalI SmaI SpeI XbaI XhoI

*Any RE site within the insert?

• No
• Yes +$50.00

*Insert has non-compatible RE ends

• Yes
• No +$50.00

e.g. Insert/BanmHI+EcoRI, Vector/BanmHI+EcoRI

*Vector provided by Customer

• ≥ 2µg (use for digestion directly)
• < 2µg (need transformation and mini prep) +$20.00

Multiple selection

*Insert plasmid provided by Customer

• < 5µg (need transformation and mini prep) +$20.00
• ≥ 5µg (use for digestion directly)

*Sequencing

-- Please Select --No Sequencing 3'- and 5' ends of insert Plus one more sequencing +$10.00Plus two more sequencing +$20.00Plus three more sequencing +$30.00

Sequencing Primer

-- 3GEX 5GEX BGHR CMV-F EGFP-C EGFP-N M13F(-21) M13F(-41) M13F(-47) M13R Myc Neo-F Neo-R pBAD-F pBAD-R pFastBacF pFastBacR SP6 SV40-promoter SV40pA-R T3 T7 T7-EEV T7-Term V5

Your Primer(s)

Please supply your primer with your sample.

*DNA Prep

• Mini Prep (free)
• EndoFree Midi Prep +$95.00
• EndoFree Maxi Prep +$120.00
• EndoFree Mega Prep +$230.00
• EndoFree Giga Prep +$400.00

Prep Scale	Typical Yield*	Turnaround Time
Mini Prep	5-10µg	Plus 2 business days
Midi Prep	100-500µg	Plus 2 business days
Maxi Prep	0.5-1.5mg	Plus 2 business days
Mega Prep	2-4mg	Plus 2 business days
Giga Prep	7-10mg	Plus 2 business days

* Data is applicable for high copy plasmid, including pUC57-Kan and pUC57-Amp.

Comments

Maximum number of characters: 1000

Additional File

Allowed file extensions to upload: doc,docx,pdf,txt,xls,xlsx,ppt,pptx,jpg,jpeg

Gene/vector sequence, map, gel image, etc.

* Required Fields